NSCs interact with endothelial cells by extending end-feet to the blood vessels, which regulate NSC self-renewal, proliferation and differentiation by secreting soluble factors. However, aging greatly impacts vascular integrity and cerebral blood flow. We previously demonstrated for the first time that infusion of youthful blood factors, via heterochronic parabiosis, can correct age-related deterioration of blood vessels in aged mice and restore blood flow to levels seen in the young, as observed with perfusion MRI on parabiotic mice.

 

Our current  projects focus on deciphering the effect of aging and neurological diseases on the blood-brain-barrier, as well as neurovascular communication.

 

To do this, we have developed a brain-on-chip microfluidic setup. This microfluidic device consists of two channels, an upper and a lower and cells communicate between them via a porous membrane. A constant flow creates mechanical forces that mimic physiological conditions. This is particularly important in the case of NSCs which, in vivo, are in contact with the constantly moving cerebrospinal fluid in the brain.